Glyphosate disrupts sperm quality and induced DNA damage of rainbow trout (Oncorhynchus mykiss) sperm

AKÇA A., KOCABAŞ M., Kutluyer F.

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART C-TOXICOLOGY AND CARCINOGENESIS, vol.39, no.4, pp.413-422, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.1080/26896583.2021.1969180
  • Journal Name: JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART C-TOXICOLOGY AND CARCINOGENESIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.413-422
  • Keywords: Glyphosate, herbicide, rainbow trout, Oncorhynchus mykiss, IN-VITRO EXPOSURE, OXIDATIVE STRESS, ANTIOXIDANT RESPONSES, HERBICIDE ROUNDUP, BISPHENOL-A, MOTILITY, FISH, CRYOPRESERVATION, INTEGRITY, VELOCITY
  • Karadeniz Technical University Affiliated: Yes

Abstract

As a widespread pollutant, glyphosate (GLY) adversely affects the aquatic environment and can impair the reproductive ability and functions of fish. The purpose of the current study was to assess in vitro effect of GLY on rainbow trout (Oncorhynchus mykiss) sperm cells. The sperm cells were exposed to different GLY concentrations (2.5, 5, 10mg/L). Sperm motility parameters were analyzed with computer assisted sperm analysis. DNA fragmentation (%) was measured by the comet assay using fluorescence microscopy. With increased GLY concentration, sperm motility and duration decreased after exposure. DNA fragmentation (% DNA in tail) in sperm cells was higher in treatments containing GLY than control (p<0.05). Consequently, sperm cells are sensitive to low doses of GLY, and this can negatively affect natural populations.