Timber-Concrete Composite construction system consists of combining timber beam or deck and concrete with different connectors. Different fastener types are used in Timber-Concrete Composite systems. In this paper, the effects of two types of fasteners on structural behavior are compared. First, the notches were opened on timber beam, and combined with reinforced concrete slab by fasteners. This system is called as Notched Connection System. Then, timber beam and reinforced concrete slab were combined by new type designed fasteners in another model. This system is called as Notched-Slab Approach. Two laboratory models were constructed and bending tests were performed to examine the fasteners' effectiveness. Bending test results have shown that heavy damage to concrete slab occurs in Notched Connection System applications and the system becomes unusable. However, in Notched-Slab Approach applications, the damage concentrated on the fastener in the metal notch created in the slab, and no damage occurred in the concrete slab. In addition, non-destructive experimental measurements were conducted to determine the dynamic characteristics. To validate the experimental results, initial finite element models of both systems were constituted in ANSYS software using orthotropic material properties, and numerical dynamic characteristics were calculated. Finite element models of Timber-Concrete Composite systems are updated to minimize the differences by manual model updating procedure using some uncertain parameters such as material properties and boundary conditions.