In this study, we aimed to clarify the taxonomic positions of Anoxybacilluskamchatkensis DSM 14988(T) and Anoxybacillusayderensis AB04(T) using whole-genome phylogenetic analysis, biochemical and chemotaxonomic characteristics. In phylogenetic trees drawn using whole-genome sequences and 16S rRNA gene sequences, A. kamchatkensis DSM 14988(T) and A. ayderensis AB04(T) clade together and showed high sequence similarity (99.6%) based on 16S rRNA gene. The average amino acid identity, average nucleotide identity and digital DNA-DNA hybridization values between A. kamchatkensis DSM 14988(T) and A. ayderensis AB04(T) were found to be greater than the threshold values for species demarcation. Most phenotypic and chemotaxonomic features between both species were almost identical except for a few exceptions. The present results show that A. kamchatkensis DSM 14988(T) is a later heterotypic synonym of A. ayderensis AB04(T).