Phoenixin-14 reduces the frequency of interictal-like events in mice brain slices


KALKAN Ö. F., ŞAHİN Z., ÖZTÜRK H., KESER H., ABİDİN S., ABİDİN İ.

EXPERIMENTAL BRAIN RESEARCH, vol.239, no.9, pp.2841-2849, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 239 Issue: 9
  • Publication Date: 2021
  • Doi Number: 10.1007/s00221-021-06179-5
  • Journal Name: EXPERIMENTAL BRAIN RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Psycinfo, Veterinary Science Database
  • Page Numbers: pp.2841-2849
  • Keywords: Extracellular recording, Phoenixin, Epileptiform activity, Entorhinal cortex, Hippocampus, EPILEPTIFORM SYNCHRONIZATION, NEUROPEPTIDES, 4-AMINOPYRIDINE, RECEPTOR, TRANSMISSION, SECRETION, DRUGS
  • Karadeniz Technical University Affiliated: Yes

Abstract

Phoenixin-14 (PNX-14) has a wide bioactivity in the central nervous system. Its role in the hypothalamus has been investigated, and it has been reported that it is involved in the regulation of excitability in hypothalamic neurons. However, its role in the regulation of excitability in entorhinal cortex and the hippocampus is unknown. In this study, we investigated whether i. PNX-14 induces any synchronous discharges or epileptiform activity and ii. PNX-14 has any effect on already initiated epileptiform discharges. We used 350 mu m thick acute horizontal hippocampal-entorhinal cortex slices obtained from 30- to 35-day-old mice. Extracellular field potential recordings were evaluated in the entorhinal cortex and hippocampus CA1 region. Bath application of PNX-14 did not initiate any epileptiform activity or abnormal discharges. 4-Aminopyridine was applied to induce epileptiform activity in the slices. We found that 200 nM PNX-14 reduced the frequency of interictal-like events in both the entorhinal cortex and hippocampus CA1 region which was induced by 4-aminopyridine. Furthermore, PNX-14 led to a similar suppression in the total power of local field potentials of 1-120 Hz. The frequency or the duration of the ictal events was not affected. These results exhibited for the first time that PNX-14 has a modulatory effect on synchronized neuronal discharges which should be considered in future therapeutic approaches.