An investigation of the effects of chronic zonisamide, sultiam, lacosamide, clobazam, and rufinamide anti-seizure medications on foliculogenesis in ovarian tissue in prepubertal non-epileptic rats

Kart P. Ö., Gürgen S. G., ESENÜLKÜ G., Dilber B., YILDIZ N., YAZAR U., ...More

International Journal of Developmental Neuroscience, vol.82, no.5, pp.436-446, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 82 Issue: 5
  • Publication Date: 2022
  • Doi Number: 10.1002/jdn.10200
  • Journal Name: International Journal of Developmental Neuroscience
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.436-446
  • Keywords: anti-seizures medication, apoptosis, EGF, epilepsy, GDF-9, IGF-1, ovary, VALPROIC ACID, ANTIEPILEPTIC DRUGS, GROWTH, WOMEN, OXCARBAZEPINE, EPILEPSY, CARBAMAZEPINE, EXPRESSION, FEMALE
  • Karadeniz Technical University Affiliated: Yes

Abstract

© 2022 International Society for Developmental Neuroscience.We aimed to determine the morphological and histological effects of zonisamide, sultiam, lacosamide, clobazam, and rufinamide on ovarian folliculogenesis in rats. Sixty female Wistar rats were divided into six experimental groups as control, zonisamide, sultiam, lacosamide, clobazam, and rufinamide groups; control solution and drugs were administered by gavage for 90 days. The number of healthy follicles in the control group was significantly higher than in the anti-medication groups (p < 0.001), and the number of corpus luteum was significantly lower (p < 0.001). There was a significant difference in the number of TUNEL positive apoptotic follicles between the control and drug groups (p < 0.001). With EGF, IGF-1, and GDF-9 staining, a very strong immunoreaction was observed in the ovarian multilaminar primary follicle granulosa cells and oocytes in the control group compared to the drug group (p < 0.001). Long-term anti-seizure medication with zonisamide, sultiam, lacosamide, clobazam, and rufinamide from prepubertal to adulthood causes apoptosis and disruption of folliculogenesis in the ovarian follicles of nonepileptic rats.