Polyhydroxybutyrate production using a wastewater microalgae based media


RAHMAN A., PUTMAN R. J., Inan K., Sal F., SATHISH A., SMITH T., ...More

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, vol.8, pp.95-98, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8
  • Publication Date: 2015
  • Doi Number: 10.1016/j.algal.2015.01.009
  • Journal Name: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.95-98
  • Keywords: Wastewater, Microalgae, Bioproduct, Polyhydroxybutyrate, ESCHERICHIA-COLI, BIOFUELS, CONSTRUCTION, EXTRACTION, BIODIESEL, ALGAE, ACIDS
  • Karadeniz Technical University Affiliated: Yes

Abstract

Bioproduct production from wastewater microalgae has the potential to contribute to societal needs with value added chemicals. Microalgae can remediate wastewater to remove nitrogen, phosphorus, and heavy metals and can be processed to produce biofuels and bioproducts. It was previously demonstrated that recombinant Escherichia coli could produce polyhydroxybutyrates (PHBs) when cultured on a wastewater microalgae wet lipid extracted media. In this present study, microalgae were harvested from the effluent of a wastewater treatment facility via centrifugation and hydrolyzed to create a liquid medium for recombinant E. coli growth and PHB production. Standard E. coli growth media was supplemented with various concentrations of hydrolyzed algal extract to produce a maximum of 31% PHB of the E. coli dry cell weight. (C) 2015 Elsevier B.V. All rights reserved.