Effect of Atomoxetine on Mouse Isolated Vas Deferens Contractility


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Arş. Gör. MEHMET KAĞAN ALTINBAŞ

Tez Türü: Eczacılıkta Uzmanlık

Tezin Yürütüldüğü Kurum: Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri, Türkiye

Tez Danışmanı: Seçkin Engin

Tezin Onay Tarihi: 2024

Tezin Dili: Türkçe

Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu

Desteklendiği Program: Diğer

Özet:

Abstract Objective: Atomoxetine (ATX), a selective noradrenaline re-uptake inhibitor, is a preferred drug with sufficient efficacy and favorable safety profile for the treatment of attention-deficit hyperactivity disorder. Ejaculatory dysfunctions have been reported in the patients receiving ATX as sexual side effects, of which underlying mechanisms are largely unknown. The present study aimed to investigate the effect of ATX on mouse isolated vas deferens (VD) contractility as a potential mechanism of ATX-induced ejaculatory dysfunction. Material and Methods: Isolated organ bath studies were performed on prostatic parts of VD obtained from adult male Balb/c mice. The effect of ATX (10-6, 10-5, 3x10-5 and 10-4 M) on KCl (80 mM)-, phenylephrine (PhE, 3x10-4 M)-, adenosine 5’-triphosphate (ATP, 10-2 M)- and electrical field stimulation (EFS; 100 V, 64 Hz)-induced contractions of VD strips were evaluated in concentration dependent manner. Results: ATX at 10-6 and 10-5 did not alter the contractile responses (p > 0.05), however, higher concentrations of ATX (3x10-5 or 10-4 M) significantly inhibited the KCl-, PhE-, ATP- and EFSinduced contractions of VD strips (p < 0.05). Conclusion: The present study demonstrated for the first time that ATX decreased the contractile responses of mouse isolated VD concentration-dependently. Our results suggest that ejaculatory dysfunction might be related to the inhibitory effect of ATX on VD.