Thermus anatoliensis sp nov., a thermophilic bacterium from geothermal waters of Buharkent, Turkey

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KAÇAĞAN M., Inan K., ÇANAKÇI S., Guler H. İ., BELDÜZ A. O.

JOURNAL OF BASIC MICROBIOLOGY, vol.55, no.12, pp.1367-1373, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 12
  • Publication Date: 2015
  • Doi Number: 10.1002/jobm.201500282
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1367-1373
  • Keywords: Thermaceae, Thermus anatoliensis, Polyphasic taxonomy, DEOXYRIBONUCLEIC-ACID, LIPID-COMPOSITION
  • Karadeniz Technical University Affiliated: Yes


A Gram-stain-negative, lack of motility, catalase- and oxidase- positive bacterium (strain MT1(T)) was isolated from Buharkent hot spring in Aydin, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain was able to grow at 45-80 degrees C, pH 5.5-10.5 and with a NaCI tolerance up to 2.0% (w/v). Strain MT1(T) was able to utilize d-mannitol and l-arabinose, not able to utilize d-cellobiose as sole carbon source. 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Thermus; strain MT1(T) detected low-level similarities of 16S rRNA gene sequences (below 97%) compared with all other species in this genus. The predominant fatty acids of strain MT1(T) were iso-C-15:0 (43.0%) and iso-C-17:0 (27.4%). Polar lipid analysis revealed a major phospholipid, one major glycolipid, one major aminophospholipid, two minor aminolipids, one minor phospholipid, and several minor glycolipids. The major isoprenoid quinone was MK-8. The DNA G+C content of MT1(T) was 69.6 mol%. On the basis of a taxonomic study using a polyphasic approach, strain MT1(T) is considered to represent a novel species of the genus Thermus, for which the name Thermus anatoliensis sp. nov. is proposed. The type strain is MT1(T) (=NCCB 100425(T) =LMG 26880(T)).