Indole-3-acetic acid (IAA) at the concentrations of 10 and 100 mg L(-1) and gibberellic acid (GA(3)) at the concentrations of 200 and 500 mg L(-1) were sprayed to persimmon plants (Diospyros lotus L.) at the bloom stages. Ascorbic acid and carotenoid contents were increased significantly by 100 mg L(-1) IAA but not in others IAA concentrations. On the other hand total sugars and reducing sugars increased in seedless fruits of D. lotus sprayed with GA(3) and IAA, except for 100 mg L(-1) IAA. However, IAA and GA(3) applications decreased levels of total phenolic substances in D. lotus fruits. The greatest changes of protein levels were observed in the fruits sprayed with 500 mg L(-1) GA(3). In addition, ascorbic acid, total sugars, reducing sugar and carotenoid levels gradually increased, while the level of phenolic substances decreased during fruit development. There was a significant change in the protein content during this process. The results showed that IAA and GA(3) not only induced parthenocarpic fruit formation, but they also changed chemical content of the fruit. Therefore GA and IAA applications might be a good way to produce the high quality fruit in D. lotus.