Research Information System
9th International Entomopathogens and Microbial Control Congress, Trabzon, Turkey, 28 - 31 October 2024, pp.106
The cotton leaf roller, Haritalodes derogata (Fabricius) (Lepidoptera: Crambidae) is an important polyphagous insect species pest of many agricultural and forestry plants. Such as mainly malvaceae familiy but also cotton, cashew, bamboo, oat, jüte ant etc. Its out-breaks has result in complete defoliation of the plants, leaving a skeleton of branches. For this purpose, firstly we studied the insecticidal effects of fifteen indigenous microbial isolates including five Bacillus thuringiensis strains (BnBt, MnD, Mm2, Xd3 and Ld8) with 1x109 cfu/ml, five entomopatphogenic fungi (Metarhizium anisopliae, As2, 4GümA and Beauveria bassiana Pa4, Pa5, Hp5) with 3x107 conidia/ml and five baculoviruses ((HycuGV, LdMNPV, AcMNPV, CpMNPV and SeMNPV) with 1,92x106 PIB/ml concentrations from our culture collection against Haritalodes derogata larvae with screening tests under laboratory conditions. In screeening assay, bacterial strains BnBt caused 100% mortality 4 days after treatment, fungal strains Pa4 caused 100% mortality 10 days after treatment, Baculovirus strains HycuGV caused 100% mortality 6 days after treatment and SeMNPV caused 100% mortality 4 days after treatment. At the end of the dose trials (1.89 x 105-9 cfu/ml for bacteria, 3 x 104-8 conidia/ml for fungi, 1 X 103-7 PIB/ml for baculovirus), LC50 was determined as 5,3x106 Cfu/mL, 4,13x10-1 conidia/mL, 9,9x101 PIB/ml and 1,26x103 respectively. All these indicate that these three microbial agents can be used for biological control of the pest.
Keywords: Microbial control, Bacillus thuringiensis, Metarhizium anisopliae, Beauveria bassiana, Viruses, Insecticidal activity