The effect of erythrocyte membranes from diabetic and hypercholesterolemic individuals on human carbonic anhydrase II activity

YILDIRMIŞ S. , ALVER A. , Yandi Y. E. , DEMİR S. , ŞENTÜRK A. , Bodur A., ...More

ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, vol.122, no.1, pp.14-18, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 122 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.3109/13813455.2015.1111909
  • Page Numbers: pp.14-18


Context: Erythrocyte membranes regulate many enzyme activities, including carbonic anhydrase II (CA II). Membrane fluidity is associated with alterations in protein function and protein-protein interactions. Objective: The purpose of this study was to show the human CA II (hCA II) activity regulation by human erythrocyte membranes from diabetic and hypercholesterolemic subjects. Materials and methods: Erythrocyte membranes were obtained from diabetic, hypercholesterolemic, and healthy subjects. hCA II activity was measured using the electrometric method. Results: hCA II activity was increased in vitro by membranes from both diabetic and hypercholesterolemic patients, with hypercholesterolemic membranes exhibiting a greater increase. Conclusion: Changes in membrane composition may affect the erythrocyte membranes' capacity to increase in vitro hCA II activity.