The effect of erythrocyte membranes from diabetic and hypercholesterolemic individuals on human carbonic anhydrase II activity
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, cilt.122, sa.1, ss.14-18, 2016 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 122 Sayı: 1
- Basım Tarihi: 2016
- Doi Numarası: 10.3109/13813455.2015.1111909
- Dergi Adı: ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.14-18
- Karadeniz Teknik Üniversitesi Adresli: Evet
Özet
Context: Erythrocyte membranes regulate many enzyme activities, including carbonic anhydrase II (CA II). Membrane fluidity is associated with alterations in protein function and protein-protein interactions. Objective: The purpose of this study was to show the human CA II (hCA II) activity regulation by human erythrocyte membranes from diabetic and hypercholesterolemic subjects. Materials and methods: Erythrocyte membranes were obtained from diabetic, hypercholesterolemic, and healthy subjects. hCA II activity was measured using the electrometric method. Results: hCA II activity was increased in vitro by membranes from both diabetic and hypercholesterolemic patients, with hypercholesterolemic membranes exhibiting a greater increase. Conclusion: Changes in membrane composition may affect the erythrocyte membranes' capacity to increase in vitro hCA II activity.