Akademik Veri Yönetim Sistemi
Comptes Rendus de L'Academie Bulgare des Sciences, cilt.77, sa.11, ss.1711-1721, 2024 (SCI-Expanded)
This study investigated the relationship between ITGB3 and BRAFV600E expression in melanoma. It specifically focused on how the overexpression of BRAFV600E and Integrin β3 impacts wound healing and the TGF-β pathway. The findings provide valuable insights into melanoma progression and highlight potential therapeutic targets. Homozygous BRAFV600E-carrying A375 and heterozygous BRAFV600Ecarrying M14 melanoma cell lines were utilized. ITGB3 mRNA levels were measured in both A375 and M14 cells, while Integrin β3 protein levels were analyzed in BRAFV600E-overexpressing A375 cells. The impact of increased BRAFV600E and Integrin β3 on cell migration was assessed using an in vitro wound healing assay. Additionally, the influence of BRAFV600E and Integrin β3 overexpression on the TGF-β pathway was evaluated through luciferase reporter assays and real-time PCR. ITGB3 levels were significantly higher in A375 cells compared to M14 cells. Overexpression of BRAFV600E in A375 cells resulted in a marked increase in Integrin β3 levels. Cells overexpressing both BRAFV600E and Integrin β3 demonstrated enhanced wound healing rates and elevated TGF-β activity. These findings suggest a strong correlation between BRAFV600E expression and Integrin β3 levels in melanoma cells. This study uncovers a significant relationship between BRAFV600E expression and Integrin β3 levels in melanoma cells. The overexpression of both BRAFV600E and Integrin β3 enhances wound healing and promotes TGF-β signalling. These findings suggest that Integrin β3 may contribute to melanoma progression and poor prognosis by influencing cell migration and the TGF-β pathway. Targeting integrins, the TGF-β pathway, and BRAFV600E present potential therapeutic strategies for melanoma treatment.