Nuclear imaging potential and in vitro photodynamic activity of Boron subphthalocyanine on colon carcinoma cells

Yurt, Fatma; Arslan, Tayfun; BIYIKLIOĞLU, ZEKERİYA; Tuncel, Ayca; Ozel, Derya; Ocakoglu, Kasim

doi:10.1016/j.jddst.2020.101567

Nuclear imaging potential and in vitro photodynamic activity of Boron subphthalocyanine on colon carcinoma cells

Atıf İçin Kopyala

Yurt F., Arslan T., BIYIKLIOĞLU Z., Tuncel A., Ozel D., Ocakoglu K.

Journal of Drug Delivery Science and Technology, cilt.56, 2020 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 56
Basım Tarihi: 2020
Doi Numarası: 10.1016/j.jddst.2020.101567
Dergi Adı: Journal of Drug Delivery Science and Technology
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, EMBASE
Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier B.V.Photodynamic therapy (PDT) has been a promising clinical agent in various types of cancer in recent years. In this study, in vitro nuclear imaging and PDT potential of Es-SubPc (Boron subphthalocyanine) were evaluated in colon adenocarcinoma cell line (HT-29) and human healthy lung fibroblast cell line (WI-38). For this purpose, the Es-SubPc was labeled with 131I using the iodogen method under the optimum conditions resulting in labeled with high yield (98.9 ± 1.2%). In addition, the uptake rate of 131I-Es-SubPc was determined in HT-29 and WI-38 cell lines. In comparison to the healthy cell line, the uptake of 131I-Es-SubPc was found to be 2-fold higher in the HT-29 cell line. For PDT studies, both cells were exposed to white light at 30–90 J/cm2 in the presence of Es-SubPc. The results showed that Es-SubPc was a good PDT agent likely to be used in HT-29 cell line. As a result, Es-SubPc can be promising nuclear imaging and PDT agent for colon carcinoma.