Pollen morphology of four Matricaria species and 28 Tripleurospermum species was investigated with light microscopies (LM) and scanning electron microscopies (SEM). Pollen slides were prepared using Wodehouse technique. Measurements were based on 20 or more pollen grains per specimen. For SEM studies, dried pollen grains were transferred on aluminum stubs and coated with gold for 4 min in a sputter-coater. The pollen grains of Matricaria and Tripleurospermum are radially symmetric and isopolar. The pollen grains of the Matricaria are oblate-spheroidal with the polar axes 16.6-31.2 mu m and the equatorial axes 18.7-23.9 mu m. Tripleurospermum is oblate-spheroidal, suboblate and prolate-spheroidal with the polar axes 15.6-32.2 mu m and the equatorial axes 17.7-38.5 mu m. The pollen grains of Tripleurospermum are operculate and tricolporate. Matricaria is operculate and usually tricolporate or rarely syncolporate, tricolpate and tetracolporate. The pollen grain of both taxa shows echinate ornamentation. The spines are commonly conical with a broadened base and a tapered apical portion. The spine length varies between 1.8-4 mu m in Tripleurospermum and 2.3-3.3 mu m in Matricaria. The width of spines varies between 2.8-4.6 mu m in Tripleurospermum and 2.4-3.6 mu m in Matricaria. Inter-spinal area shows granulate-perforate, reticulate-perforate, rugulate-perforate ornamentations and the tectum surrounding the spine base is micro perforate. Overall exine thickness ranges from 2.8 to 4.8 mu m in Tripleurospermum, 3.6 to 5.2 mu m in Matricaria. Intine is thicker under pores in Tripleurospermum (0.3-0.62 mu m) than in Matricaria (0.6-0.8 mu m). Inter-spinal ornamentations, pollen shape and the numbers of perforations at the spin base have been observed as important morphological characters.