Fish fauna in the upper and middle Ceyhan River basin were investigated with the distribution and systematical determination from May 2001 to April 2004. The fish specimens were obtained by electro fishing and gill nets from two lakes, two reservoirs and 18 streams. A total of 2,414 specimens were collected and 1,156 of these were investigated diagnostic characteristics. Twenty species belong to 10 families were determined. The fish species determined in the study are: Anguilla anguilla, Salmo trutta macrostigma, Cyprinus carpi, Acanthobrama sp., Alburnus orontis, Pseudophoxinus zekayi, Squalius kottelati, Garra rufa, Chondrostoma regain:, Luciobarbus pectoralis, Capoeta angorae, Capoeta erhani, Cobitis evreni, Schistura ceyhanensis, Oxynemacheilus sp., Silurus gariepinus, Aphanius menu), Gambusia affinis and Salaria fluviatilis. Endemic species Pseudophoxinus zekayi, Capoeta erhani, Schistura ceyhanensis and Cobitis evreni reported in recent years were described.