Tumor-selective new piperazine-fragmented silicon phthalocyanines initiate cell death in breast cancer cell lines

SARI C., NALÇAOĞLU A., DEĞİRMENCİOĞLU İ., Eyupoglu F.

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, cilt.216, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 216
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.jphotobiol.2021.112143
  • Dergi Adı: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Breast neoplasms, Photodynamic therapy, Silicon phthalocyanine, Piperazine, Reactive oxygen species, PHOTODYNAMIC THERAPY, POLY(ADP-RIBOSE) POLYMERASE, ZINC(II) PHTHALOCYANINE, OXIDATIVE STRESS, PASSAGE NUMBER, APOPTOSIS, PATHWAYS, PHARMACOKINETICS, PHOTOSENSITIZERS, LOCALIZATION
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

A new silicon phthalocyanine with piperazine-furan ring and its quaternized form were synthesized. All compounds were analyzed by spectroscopic techniques (FT-IR, H-1-NMR, MS, and UV-vis), and the absorbance characteristics of silicon phthalocyanines were evaluated with the expected strong typical absorption bands in the far-red spectrum. The cytotoxic effects of these phthalocyanines induced by photodynamic therapy (PDT) were determined in a dose-dependent manner. Following cytotoxicity analysis, flow cytometric research of cell death was performed. The formation of reactive oxygen species (ROS) was determined by confocal microscopy. High levels of cytotoxicity and decreased viable cell population have been detected in cancer cells after treatment. In addition, ROS formation was observed in PDT treated cancer cells. However, low levels of cell death and ROS formation were observed in non-tumorigenic cells. According to western blot data, PDT-mediated treatment was found to provide different expression patterns of the cleaved PARP1 protein. The presented study demonstrates that PDT-mediated treatment of newly synthesized phthalocyanines has significant anti-cancer effects on breast cancer cells and may induce different cell death pathways.