Akademik Veri Yönetim Sistemi
7th International Symposium on Advances in Pharmaceutical Analysis (APA 2025) , Ankara, Türkiye, 24 - 27 Ağustos 2025, ss.102, (Özet Bildiri)
Breast cancer is defined as a hormonal and heterogeneous disease with various morphological subgroups, and it is the most prevalent cancer among women globally [1]. Natural products, especially medicinal plants, have historically been an essential source of raw materials in anticancer drug development studies [2]. The genus Omphalodes Moench (Boraginaceae) comprises 5 species in the flora of Türkiye and includes 25 herbaceous species worldwide [3,4]. Previous research has indicated that plants in the Boraginaceae family exhibit cytotoxic properties on breast cancer [5,6]. There are no studies assessing the therapeutic value of Omphalodes cappadocica (Willd.) DC., as a study material for breast cancer has been found in the literature. This study aims to evaluate the possible cytotoxic properties of a methanol extract prepared from the aerial parts of O. cappadocica on breast cancer cells and to analyze its phytochemical composition.
Specimens of the aerial parts of O. cappadocica were collected from Gündoğan, Köprübaşı, and Trabzon (Herbarium Registration number: HUEF24213). The pulverized aerial parts were macerated with methanol for 8 hours at room temperature, followed by filtration. The filtrate was evaporated to dryness in a rotary evaporator at 40 °C. The effect of crude methanol extract was assessed in estrogen receptor-positive MCF-7 (ATCC® HTB-22TM) and estrogen receptor- negative MDA-MB-231 (ATCC® HTB-26TM) breast cancer cell lines with the MTT (3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide) assay. The extract's selective cytotoxicity has been examined using healthy mouse fibroblast L929 (ATCC® CCL-1) cells. A Shimadzu-Nexera ultrahigh-performance liquid chromatograph paired with a tandem mass spectrometer was employed for the quantitative assessment of 53 phytochemicals.
As a result, after 48 hours of incubation, the methanol extract showed a selective cytotoxic effect on MCF-7 and MDA-MB-231 cells, with IC50 values of 14.08±0.05 μg/ml and 10.63±0.01 μg/ml, respectively. The selectivity index of the extract was calculated as 5.15 and 6.82 for MCF-7 and MDA-MB-231 cells, respectively. LC-MS/MS analysis revealed that the major components in the methanol extract were rosmarinic acid (97.095 mg/g), quinic acid (48.987 mg/g), and vanillic acid (10.009 mg/g). A previous investigation analyzed the components of the essential oil of O. cappadocica by gas chromatography and assessed its antibacterial activity [7]. This study is the first investigation testing the cytotoxic properties of the aerial parts of O. cappadocica, and analyzing its phenolic composition. The study's findings indicated that O. cappadocica exhibits cytotoxic activity in both cell lines with selective toxicity. The effect may result from the abundance of phenolic compounds in the aerial parts of the plant. To clarify this situation, studies on the isolation and structural characterization of secondary metabolites responsible for the active methanol extract may be performed.