The metal content of Colorado potato beetles elytra in Turkey was determined using the energy dispersive X-ray fluorescence method. A radioisotope excited X-ray fluorescence analysis using the method of standard addition is applied for the elemental analysis of Colorado potato beetles elytra. An annular 50 mCi Am-241 radioactive source and an annular 50 mCi Fe-55 radioactive source were used for excitation of characteristic K X-rays. An Si(Li) detector which has a 147 eV full-width at half-maximum for 5.9 keV photons was used for intensity measurements. The procedure of sample preparation and the results of analysis were presented. (c) 2005 Elsevier Ltd. All rights reserved.