The European grapevine moth, Lobesia botrana (Denis & Schiffermuller) (Lepidoptera: Tortricidae) is one of the most harmful insect pests of grape species in many countries. The purpose of this study was to find a safe and effective microbial control agents against L. botrana. Pathogenicity of 4 bacterial, 12 fungal and 4 viral agents was tested on the 3rd instar larvae of L. botrana. Results showed that one strain of Bacillus thuringiensis (MnD) had the highest mortality (100%) on the larvae within 3 days of treatment. On the other hand, 93% mortality and mycosis with Metarhizium anisopliae (Gg-12) and 90% mortality with Chilo iridescent virus (CIV) were determined on the larvae of the pest. The findings indicate that B. thuringiensis (MnD), M anisopliae (Gg-12) and C. iridescent virus (CIV) are considered suitable microbial control agents against L. botrana larvae.