Akademik Veri Yönetim Sistemi
33rd International Symposium on Pharmaceutical and Biomedical Analysis, Ankara, Türkiye, 2 - 06 Temmuz 2023, ss.88-89
The use of deep eutectic solutions as alternative solvents, which has attracted a great deal of attention in recent years and is one of the subjects of interest in green chemistry, has come to the fore in research. In this study, synthesized natural deep eutectic solvent (NADES) (p-coumaric acid- tert-butyl ammonium bromide) were used for pretreatment. Pretreatment was carried out in a microwave oven. Essential oil extraction was performed with the hydrodistillation system combined with a microwave oven (MHD). While the yield of essential oil analysis without pretreatment was 19% and 21%, respectively, the results were obtained as 23% and 22% in pretreated ones. While the number of essential oil components without pretreatment was 20 and 27, the number of compounds obtained with pretreatment was determined as 52 and 57. In the analysis without pretreatment, the major compounds γ-Terpinen (10.69%) and Terpinen-4-ol (31.32%) were found for Origanum micranthum, Linalool (13.85%) and α-Terpineol (24.61%) for Origanum minutiflorum. In the pre-treated analysis, the major compounds Terpinen (13.02%) and Terpinen-4-ol (14.23%) were found for Origanum micranthum, Linalool (19.96%) and α-Terpinyl acetate (22.71%) for Origanum minutiflorum. Antioxidant activities of all essential oils obtained from two plants were analyzed by DPPH, FRAP and CUPRAC tests. It was observed that the oils obtained from pretreated plants had higher antioxidant activity compared to FRAP and CUPRAC analyzes, but DPPH analyzes showed lower activity. Considering all, NADES had a significant impact on the extraction of essential oil with higher yield, and more quantity, especially when it was combined with microwave technology. Moreover, the NADES-MHD as an economical and eco-friendly technique had the potential to be applied to other plant materials.