This study was conducted in order to investigate the vegetative compatibility groups (VCGs) and the pathogenicity of Verticillium dahliae isolates from strawberry plants in Erzurum and Erzincan provinces during 2008-2010. As a result of isolations, two hundred two V. dahliae isolates were isolated from strawberry plants. The vegetative compatibility groups of the isolates were determined using nitrate-nonutilizing (nit) mutants. A total of 881 mutants were produced from the isolates, and classified as nit1 (781 mutants) and nitM (100 mutants). As a result of complementation test with international tester isolates, 148 isolates were assigned to VCG2B, 32 isolates to VCG4B and 13 isolates to VCG2A, whereas 9 isolates were incompatible with tester isolates. Pathogenicity experiments were tested on strawberry (cv. Fern) by the root-tip method. It was identified that VCG2A isolates were more aggressive than VCG4B and VCG2B isolates. This is the first report of VCGs of V. dahliae isolates from strawberry plants in Turkey.