Detection of nimesulide metabolites in rat plasma and hepatic subcellular fractions by HPLC-UV/DAD and LC-MS/MS studies

KÜÇÜKGÜZEL İ., KÜÇÜKGÜZEL İ., Oral B., Sezen S., Rollas S.

EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS, vol.30, pp.127-134, 2005 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30
  • Publication Date: 2005
  • Doi Number: 10.1007/bf03226418
  • Journal Name: EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.127-134
  • Keywords: nimesulide, S9 fraction, nitroreduction, nitroarenes, LC-MS/MS, DIFLUNISAL ACYL GLUCURONIDE, LIQUID-CHROMATOGRAPHY, LIVER-MICROSOMES, HYDROXYNIMESULIDE, DISPOSITION, REDUCTION, IV
  • Karadeniz Technical University Affiliated: No

Abstract

Nimesulide (4-nitro-2-phenoxymethanesulfonanilide) is an atypical NSAID lacking a carboxylic acid moiety. It has a good gastric tolerability due to selective inhibition of COX-2. The study objectives in the present work were to characterize the metabolism of nimesulide in rat plasma at certain time intervals. In vitro studies were also carried out to examine if nitroreduction takes place in vitro using rat hepatic subcellular fractions (microsomal and S9 fraction) besides aromatic hydroxylation. This communication describes detection and characterization of nimesulide metabolites isolated from plasma and hepatic subcellular post-incubates by the use of HPLC-UV/diode array and LC-MS/MS. Hydroxynimesulide was the major metabolite both in vivo and in vitro whereas nitroreduction was observed only in vitro with subcellular fractions under anaerobic conditions.