BIOELECTROCHEMISTRY, cilt.160, ss.1-11, 2024 (SCI-Expanded)
Herein, a comparative study between novel water-soluble phthalocyanine-based biosensors was performed for
the application of glucose sensing. For this purpose, two different copper (II) and manganese (III) phthalocyanines and their water-soluble derivatives were synthesized, and then their role as a supporting material for
enzyme immobilization was evaluated by comparing their sensor performances. Two different phthalocyanine
(AP-OH2-MnQ (MnPc) and AP-OH2-CuQ (CuPc)) were tested using electrochemical biosensor with immobilized
glucose oxidase (GOx). To the best of our knowledge, the related water-soluble phthalocyanine-based glucose
biosensors were attempted for the first time, and the developed approach resulted in improved biosensor
characteristics. The constructed biosensors GE/MnPc/GOx and GE/CuPc/GOx showed good linearity between
0.003–1.0 mM and 0.05–0.4 mM, respectively. The limit of detection was estimated at 0.0026 mM for the GE/
MnPc/GOx and 0.019 mM for the GE/CuPc/GOx. KM
app and sensitivity values were also calculated as 0.026 mM
and 175.043 µAmM− 1 cm− 2 for the GE/MnPc/GOx biosensor and 0.178 mM and 117.478 µAmM− 1 cm− 2 for the
GE/CuPc/GOx biosensor. Moreover, the fabricated biosensors were successfully tested to detect glucose levels in
beverages with high recovery results. The present study shows that the proposed water-soluble phthalocyanines
could be a good alternative for quick and cheap glucose sensing with improved analytical characteristics.