Synthesis and comparison of the performance of two different water-soluble phthalocyanine based electrochemical biosensor

Osmanoğulları S. C., Öztürmen B. A., Bekircan O., Bıyıklıoğlu Z., Söylemez S.

BIOELECTROCHEMISTRY, cilt.160, ss.1-11, 2024 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 160
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1016/j.bioelechem.2024.108788
  • Dergi Adı: BIOELECTROCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-11
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Herein, a comparative study between novel water-soluble phthalocyanine-based biosensors was performed for the application of glucose sensing. For this purpose, two different copper (II) and manganese (III) phthalocyanines and their water-soluble derivatives were synthesized, and then their role as a supporting material for enzyme immobilization was evaluated by comparing their sensor performances. Two different phthalocyanine (AP-OH2-MnQ (MnPc) and AP-OH2-CuQ (CuPc)) were tested using electrochemical biosensor with immobilized glucose oxidase (GOx). To the best of our knowledge, the related water-soluble phthalocyanine-based glucose biosensors were attempted for the first time, and the developed approach resulted in improved biosensor characteristics. The constructed biosensors GE/MnPc/GOx and GE/CuPc/GOx showed good linearity between 0.003–1.0 mM and 0.05–0.4 mM, respectively. The limit of detection was estimated at 0.0026 mM for the GE/ MnPc/GOx and 0.019 mM for the GE/CuPc/GOx. KM app and sensitivity values were also calculated as 0.026 mM and 175.043 µAmM− 1 cm− 2 for the GE/MnPc/GOx biosensor and 0.178 mM and 117.478 µAmM− 1 cm− 2 for the GE/CuPc/GOx biosensor. Moreover, the fabricated biosensors were successfully tested to detect glucose levels in beverages with high recovery results. The present study shows that the proposed water-soluble phthalocyanines could be a good alternative for quick and cheap glucose sensing with improved analytical characteristics.