Characterization of Pseudoalteromonas sp. from Aquaculture Environment and Optimization of Fermentation Culture Parameters by RSM-Based Modeling

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Yaylacı E. U.

Turkish Journal of Fisheries and Aquatic Sciences, vol.22, no.11, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 11
  • Publication Date: 2022
  • Doi Number: 10.4194/trjfas21726
  • Journal Name: Turkish Journal of Fisheries and Aquatic Sciences
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Keywords: Pseudoalteromonas, Anti-Vibrio activity, Response surface optimization, BOX-BEHNKEN DESIGN, BACILLUS SPP., SP NOV., MARINE, BACTERIUM, ALTEROMONAS, INFECTIONS, RESISTANCE, PROBIOTICS, PROPOSAL
  • Karadeniz Technical University Affiliated: Yes


© 2022, Central Fisheries Research Inst. All rights reserved.The aim of the study was to identify Pseudoalteromonas strain OS-9 and evaluate its anti-Vibrio potential. Based on the morphological, biochemical, and 16S rRNA sequence analysis, the strain was identified as Pseudoalteromonas haloplanktis. Experiments designed with Box Behnken showed that cell density and cell-free supernatant activity were simultaneously maximum at 30°C, pH 7, and 90 h. The coefficient values obtained from the response surface methodology regression equations determined that pH was more effective on responses than other physical variables. The cell-free supernatant of P. haloplanktis OS-9 extracted under optimized conditions was only effective on Gram-negative fish pathogenic bacteria and especially on Vibrio. Among Vibrio spp., the strongest inhibitory effect was observed against V. rotiferianus. At the maximum cell density (10.48 OD), the strain OS-9 showed the maximum inhibitory effect against V. rotiferianus (26.42 mm zone diameter). In co-culture assay, the growth of V. rotiferianus was inhibited after 96 h with an initial level of 1.0 × 106 CFU ml-1 by the strain OS-9. These results indicated that P. haloplanktis strain OS-9 could be considered as a potential Vibrio bio-controlling agent.