Black alder (Alnus glutinosa subsp. barbata) has a wide natural distribution area in Eastern Black Sea Region of Turkey. This species is important due to its growth rate, contributions to soil reclamation, and its usage in forestry products. Anatomical characteristics of alder woods of two different sites (Trabzon and Giresun) from the Eastern Black Sea Region were compared. Nine sample areas were selected in Akcaabat region and 8 in Espiye region. A tree which had dominant height was sawn (cut down) from these sample areas. Wood samples were taken from diameter at breast height of these sawn trees to determine anatomical characteristics, such as tangential diameter of vessel, radial diameter of vessel, vessel cell length, number of vessels in 1 mm(2), number of rays in 1 mm, ray height, ray width, fiber length, fiber width, lumen diameter of fiber, and thickness of fiber. On sampling sites, soil profiles were excavated and soil samples were taken from fixed depth levels (0-10 cm, 10-30 cm. 30-50 cm, 50-80 cm, and 80-120 cm). Soil particle size analysis was made on collected soil samples to determine soil type and sand, silt and clay content of soil. Soil pH, soil organic carbon content and available water capacity were also measured. SPSS statistical packet software was used to determine variances (ANOVA) in anatomical wood characteristics of alder between study sites. Analysis of variance results showed that number of vessels in 1 mm2, ray width, vessel cell length, fiber length, fiber width, and lumen width showed significant differences (p<0.001) in sample woods from different study areas. These differences in anatomical wood characteristics were caused by different micro-environmental (soil properties and rainfall) conditions.