Primula x uzungolensis Terzioglu & Coskuncelebi, a natural hybrid from north-eastern Anatolia, is described and illustrated with its parents. Based on its morphology and molecular characteristics, we conclude that P x uzungolensis is a new natural hybrid between P. acaulis (L.) Hill subsp. rubra (Sm.) Greuter & Burdet and P. veris L. subsp. columnae (Ten.) Maire & Petitmengin. Maximum parsimony (MP) analysis based on nuclear ribosomal DNA (nrDNA) and chloroplast DNA (cpDNA) shows that P. veris subsp. columnae is the maternal parent of the putative hybrid, and the hybridisation between P. veris subsp. columnae and P. acaulis subsp. rubra is unidirectional.