A new microsporidium, Vairimorpha subcoccinellae n. sp (Microsporidia: Burenellidae), isolated from Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae)

Baki H., Bekircan C.

JOURNAL OF INVERTEBRATE PATHOLOGY, vol.151, pp.182-190, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 151
  • Publication Date: 2018
  • Doi Number: 10.1016/j.jip.2017.12.004
  • Journal Name: JOURNAL OF INVERTEBRATE PATHOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.182-190
  • Keywords: Coccinellid, Galega officinalis, Microsporidia, Vairimorpha, CONVERGENT LADY BEETLE, MOLECULAR CHARACTERIZATION, PROTOZOA-MICROSPORIDA, NECATRIX KRAMER, NOSEMA-ALGERAE, PATHOGEN, LEPIDOPTERA, CNIDOSPORA, GENUS
  • Karadeniz Technical University Affiliated: Yes

Abstract

A new microsporidium was isolated from Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae), a pest of Galega officinalis L. in Turkey. Infection in larval and adult stages was systemic with mature spores produced in the midgut, gonads, Malpighian tubules and, most extensively, fat body tissues. The microsporidium was polymorphic with two sporulation sequences producing two types of spores, binucleate spores with 13-15 coils of the polar tube, and uninucleate spores with 7 coils of the polar tube that developed within a sporophorous vesicle (SPV) to form meiospores. The 16S small subunit rRNA (SSU rRNA) gene of the microsporidium was sequenced and compared with twenty-seven microsporidian sequences from GenBank. Based on the phylogenetic analysis of the SSU rRNA sequence, this microsporidium is unique within the Vairimorpha group. Morphological and genetic characters indicate that the described microsporidium is dissimilar to all known Vairimorpha species, and so is named here as Vairimorpha subcoccinellae n. sp.