Cloning, Expression and Characterization of Alkaline Phosphatase from a Thermophilic Bacterium Geobacillus caldoxylosilyticus TK4

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COL M., ÇOLAK A., Ertunga N., Yildirim M., Ozel A.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.35, no.3, pp.208-214, 2010 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 35 Issue: 3
• Publication Date: 2010
• Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
• Page Numbers: pp.208-214
• Keywords: Alkaline phosphatase, Gene expression, Geobacillus caldoxylosilyticus, Cloning, Thermophilic, ANOXYBACILLUS-GONENSIS G2, PURIFICATION, ENZYME, ISOZYMES, CLEAVAGE
• Karadeniz Technical University Affiliated: Yes

Abstract

Aim: To clone the gene for alkaline phosphatase (AP) from a novel thermophilic Geobacillus caldoxylosilyticus TK4 strain and to express and characterize this enzyme.