Phenolic-Rich <i>Aronia melanocarpa</i> (cv. Nero) Extract Attenuates Doxorubicin-Induced Nephrotoxicity Associated with Reduced Oxidative Stress, Inflammation, and Endoplasmic Reticulum Stress
MOLECULES, cilt.31, sa.20, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31 Sayı: 20
- Basım Tarihi: 2026
- Doi Numarası: 10.3390/molecules31203732
- Dergi Adı: MOLECULES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE, Directory of Open Access Journals, Academic Search Ultimate (EBSCO), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest)
- Karadeniz Teknik Üniversitesi Adresli: Evet
Özet
Aronia melanocarpa (black chokeberry) is a polyphenol-rich berry recognized as a functional food because of its potent antioxidant properties. This study investigated the phenolic composition, antioxidant capacity, and nephroprotective effects of A. melanocarpa extract (AME) against doxorubicin (DXR)-induced renal injury. Antioxidant activity was evaluated by determining total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. Thirty-six female Sprague-Dawley rats (180-220 g) were randomly allocated to four groups (n = 9 per group): Control, AME, DXR, and AME + DXR. AME (200 mg/kg/day) was administered orally for 21 consecutive days as a pretreatment, and acute nephrotoxicity was induced by a single intraperitoneal injection of DXR (20 mg/kg) on day 21. Samples were collected 24 h after DXR administration. Renal injury was evaluated using biochemical, histopathological, oxidative stress (OS), inflammatory, endoplasmic reticulum stress (ERS), and apoptosis-related parameters, together with TUNEL staining. HPLC-PDA analysis of 29 targeted phenolic compounds identified cya-nidin-3-galactoside (2440 & micro;g/g FW), cyanidin-3-arabinoside (350 & micro;g/g FW), chlorogenic acid (251.83 & micro;g/g FW), and rutin (32.29 & micro;g/g FW) as the major constituents. DXR markedly impaired renal function and increased oxidative stress, ERS, inflammation, and apoptosis, whereas AME pretreatment significantly attenuated these alterations. These findings are consistent with a protective effect of phenolic-rich AME against acute DXR-induced renal injury in rats, associated with attenuation of oxidative stress, inflammation, ERS, and apoptosis. Further studies are needed to confirm these findings and evaluate their potential clinical relevance.