Tetra-aldehyde and ketone derivatives (2a-d) which were used to obtain tetra thiosemicarbazone compounds 3a-h were synthesized via the reaction of Ethene-1,1,2,2,-tetra-yl-tetra-methylene-tetra-bromide (1) with aldehydes and ketons. Then, tetra-thiosemicarbazone compounds 3a-h were obtained from the reactions of tetra-aldehyde and keton derivatives (2a-d) with thiosemicarbazite and 4-methyl thiosemicarbazite, respectively. In the same way, tetra-4-methyl-5-ethoxycarbonyl-2,3-dihydro-1,3-thiazole compounds 4a-h were synthesized via the reaction of tetra-thiosemicarbazone compounds 3a-h with ethyl-2-chloroacetoacetate. Then, tetra-thiosemicarbazone compounds 3a-h were reacted with acetic anhydride, and yielded tetra-2-acetyl-amino-4-acetyl-4,5-dihydro-1,3,4-thiodiazol compounds (5a,b,c,e,f,g). They were characterized by elemental analysis, infrared, H-1 and C-13-NMR, mass spectroscopy. Then, the microbial features of all compounds were studied by the known method.