Neogobius rizensis sp. nov. and Neogobius turani sp. nov. are described from freshwater streams in northeastern Turkey. The new species are assigned to Neogobius and the subgenus Ponticola based on diagnostic features belonging to these taxa. Neogobius rizensis differs from all other species of the subgenus Ponticola by the following combination of characters: snout 1.6-1.8 orbit of eye, upper lip swollen toward angle of jaws and wide 0.9-1.3 lateral preorbital area, angle of jaws ending below anterior edge of eye and caudal peduncle depth 0.5-0.6 own length. Neogobius turani is distinguished by a following combination of characters: head width 1.5-1.6 own depth, snout 1.4-1.5 orbit of eye, upper lip not expanded or slightly expanded and wide 0.7-0.8 of lateral preorbital area, angle of jaws ending below pupil.