Decreased Na+/K+ pump activity in the erythrocyte membrane due to malondialdehyde in rheumatoid arthritis: an in vivo and in silico study

Oğul Y., GÜR F., Gür B., Cengiz M., SARI R. A., KIZILTUNÇ A.

Canadian Journal of Physiology and Pharmacology, vol.100, no.10, pp.968-982, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 100 Issue: 10
  • Publication Date: 2022
  • Doi Number: 10.1139/cjpp-2022-0171
  • Journal Name: Canadian Journal of Physiology and Pharmacology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, MEDLINE, SportDiscus, Veterinary Science Database
  • Page Numbers: pp.968-982
  • Keywords: Na+/K+-ATPase activity, rheumatoid arthritis, lipid peroxidation, negative binding energy, molecular docking, SUPEROXIDE-DISMUTASE, ANTIOXIDANT STATUS, SODIUM, PATHOGENESIS, DOCKING, ELUCIDATION, INHIBITION, OXIDATION, CATALASE
  • Karadeniz Technical University Affiliated: No

Abstract

© 2022 The Author(s).Apart from demonstrating the interaction behavior of malondialdehyde (MDA) with Na+/K+-ATPase using in silico, the current study aims to investigate the effect of rheumatoid arthritis-related oxidative stress on Na+/K+-ATPase activity that is present in the erythrocyte cell membrane, which is rich in proteins vulnerable to damage from MDA and other free radicals. The target population of this study consists of 28 rheumatoid arthritis patients and 20 healthy volunteers whose MDA levels and Na+/K+-ATPase activity were determined. It was shown that MDA levels of rheumatoid arthritis patients increased (p < 0.001) and their Na+/K+-ATPase activity noticeably decreased when compared to those of healthy individuals. Also, according to this in silico modeling, MDA decreased Na+/K+-ATPase activity in line with the correlation analyses. Consequently, while elevated levels of MDA in the rheumatoid arthritis group were suggestive of oxidative stress, a decreased Na+/K+-ATPase-activity led us to speculate that the cellular membrane had sustained injury. Therefore, our results could be useful in explaining how MDA affects Na+/K+-ATPase activity in the interior of a specific molecular pathway.