Cities have existed throughout the history as a combination and conflict of various cultures. The values of citizens are shaped by cultural accumulation that is created from the past to our times. Cultural elements cover a wide scope of fields from science in life to art. The history of the city helps the recognition of the city's architecture, music, tradition, habits and culture and creates the identity of the city. All of these elements that form a city constitute the cultural heritage of the city. The best witnesses of cultural heritage are city museums which present cities as they are. City museums are one of archive sources that contain all the information that may be gathered about a city. Museums are significant structures that transfer the past to the future, witness all the times experienced by cities and symbolize cities. This study was form around who the structure and culture of a city is reflected by museums, which are the strongest protectors of the cultural heritage of a city. This is why this study discusses the province of Trabzon which has hosted several civilizations from the past to the present and protected its cultural heritage, as well as its city museums. Information and documents were collected in relation to the city museums in the province of Trabzon, and city museums were discussed with the method of field surveys. The city of Trabzon has been covered in the scope of the study with the city's museums where it preserves its cultural heritage. The museum contributes to the development and strengthening of the social consciousness as well as the transfer of the city's values, and the values we make us with great care. The purpose of the study is to reveal the extent to which city museums protect the cultural heritage of the city and transfer it to future generations.