Kribbella sindirgiensis sp nov isolated from soil


Ozdemir-Kocak F., Isik K., Saricaoglu S., Saygin H., Inan-Bektas K. , Cetin D., ...More

ARCHIVES OF MICROBIOLOGY, vol.199, no.10, pp.1399-1407, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 199 Issue: 10
  • Publication Date: 2017
  • Doi Number: 10.1007/s00203-017-1414-x
  • Title of Journal : ARCHIVES OF MICROBIOLOGY
  • Page Numbers: pp.1399-1407
  • Keywords: Kribbella sindirgiensis, Nocardioidaceae, Polyphasic taxonomy, 16S rRNA gene, SEQUENCE-ANALYSIS, DNA, CLASSIFICATION

Abstract

A Kribbella strain FSN23(T) was isolated from soil sample which was collected from Caygoren Dam lakeside located in SA +/- ndA +/- rgA +/-, Turkey. The isolate was investigated using a polyphasic approach consisting of numeric, chemotaxonomic and molecular analysis. The isolate indicated chemotaxonomic, morphological and phylogenetic properties associated with members of the genus Kribbella. Phylogenetic analysis based on the 16S rRNA sequence of the strain demonstrated that the strain forms a subclade with K. aluminosa HKI 0478(T) and K. jejuensis HD9(T). The organism formed an extensively branched substrate and aerial hyphae which generated spiral chains of spores with smooth surfaces. The cell wall contained ll-diaminopimelic acid, and the whole cell sugars were glucose and ribose along with trace amounts of mannose. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids and five unidentified polar lipids. The predominant menaquinone was MK-9(H-4). The major cellular fatty acids were anteiso-C-15:0 and iso-C-16:0. Polyphasic taxonomy properties confirm that strain FSN23(T) represents a novel Kribbella taxon distinguished from closely related type strains. Hence, strain FSN23(T) (=KCTC 29220(T) = DSM 27082(T)) is proposed as the type strain of a novel species with the name Kribbella sindirgiensis sp. nov.