Isolation of the Major Secondary Metabolites and Biological Activities of the Aerial Parts of Trachystemon orientalis


Badem M., Subaş T., Kanbolat Ş., Özgen U., Şener S. Ö., Yazıcı G., ...More

The 10th International Mediterranean Symposium on Medicinal and Aromatic Plants, İstanbul, Turkey, 25 - 27 April 2024, pp.133

  • Publication Type: Conference Paper / Summary Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.133
  • Karadeniz Technical University Affiliated: Yes

Abstract

Trachystemon orientalis D. Don (Boraginaceae) is a perennial plant, which is used as food and for treatment of the various diseases [1,2]. Studies have shown that its phytochemical content includes anthocyanins, proanthocyanidin, phenolic acids, essential oil, tannins, mucilage, saponin, minerals, and vitamins [3]. In this study, it was aimed to performed isolation and biological activity (antioxidant and lipase inhibitory activities) studies on the extracts of the aerial parts of T. orientalis. The structures of the isolated compounds were characterized using 1H-NMR, 13C-NMR, and 2D-NMR spectral methods. Ferric reducing antioxidant power (FRAP) assay to determine antioxidant activity, and pancreatic lipase enzyme inhibitory activities of the extracts were evaluated. β-sitosterol (1) was isolated from the chloroform subextract. Besides, rosmarinic acid (2) from the ethyl acetate subextract, the mixture of rosmarinic acid and danshensu (3) from the remaining aqueous subextract were isolated. The compounds were obtained for the first time from the aerial parts of the plant with the study. According to the results of biological activity assays, the ethyl acetate subextract showed the highest FRAP value (TEAC = 177 ± 4.5825 µM) and pancreatic lipase inhibitory activity (IC50 = 47.577 ± 0.931 µg/mL). Rosmarinic acid, the main component of the ethyl acetate subextract, may be responsible for the high activities. According to the results of this study, T. orientalis has high antioxidant capacity and lipase inhibitory activity and may be a promising potential natural agent for obesity.