Akademik Veri Yönetim Sistemi
The 10th International Mediterranean Symposium on Medicinal and Aromatic Plants, İstanbul, Türkiye, 25 - 27 Nisan 2024, ss.133
Trachystemon orientalis D. Don (Boraginaceae) is a perennial plant, which is used as food and for
treatment of the various diseases [1,2]. Studies have shown that its phytochemical content includes
anthocyanins, proanthocyanidin, phenolic acids, essential oil, tannins, mucilage, saponin, minerals,
and vitamins [3]. In this study, it was aimed to performed isolation and biological activity (antioxidant
and lipase inhibitory activities) studies on the extracts of the aerial parts of T. orientalis. The structures
of the isolated compounds were characterized using 1H-NMR, 13C-NMR, and 2D-NMR spectral
methods. Ferric reducing antioxidant power (FRAP) assay to determine antioxidant activity, and
pancreatic lipase enzyme inhibitory activities of the extracts were evaluated. β-sitosterol (1) was
isolated from the chloroform subextract. Besides, rosmarinic acid (2) from the ethyl acetate
subextract, the mixture of rosmarinic acid and danshensu (3) from the remaining aqueous subextract
were isolated. The compounds were obtained for the first time from the aerial parts of the plant with
the study. According to the results of biological activity assays, the ethyl acetate subextract showed
the highest FRAP value (TEAC = 177 ± 4.5825 µM) and pancreatic lipase inhibitory activity (IC50 =
47.577 ± 0.931 µg/mL). Rosmarinic acid, the main component of the ethyl acetate subextract, may be
responsible for the high activities. According to the results of this study, T. orientalis has high
antioxidant capacity and lipase inhibitory activity and may be a promising potential natural agent for
obesity.