The level of ductility is determined by depending on the intended use of the building, the region's seismic characteristics and the type of structural system when buildings are planned by engineers. Major portion of seismic energy is intended to be consumed in the plastic zone in structural systems of high ductility, so the occurrence of damages in load bearing and non-load bearing structural elements is accepted in planning stage under severe earthquakes. However, these damages must be limited among specific values in order not to endanger buildings in terms of the bearing capacity. Isolators placed between the basement and upper structure make buildings behave elastically by reducing the effects of seismic loads and improving seismic performance of building significantly. Thus, damages can be limited among desired values. In this study, the effectiveness of seismic isolation is investigated on both fixed based and seismic isolated models of a hospital building with high ductility level with regard to lateral displacements, internal forces, structural periods and cost of the building. Layered rubber bearings are interposed between the base of the structure and foundation. Earthquake analysis of the building are performed using earthquake records in time domain (Kocaeli, Loma Prieta and Landers). Results obtained from three-dimensional finite element models are presented by graphs and tables in detail. That seismic isolation reduces significantly the destructive effects of earthquakes on structures is seen from the results obtained by seismic analysis.