Protein-protein interactions among the structural proteins of Chilo iridescent virus

ÖZŞAHİN E., van Oers M. M., NALÇACIOĞLU R., DEMİRBAĞ Z.

JOURNAL OF GENERAL VIROLOGY, cilt.99, sa.6, ss.851-859, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 99 Sayı: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1099/jgv.0.001067
  • Dergi Adı: JOURNAL OF GENERAL VIROLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.851-859
  • Anahtar Kelimeler: Chilo iridescent virus, structural proteins, protein-protein interaction, yeast-two-hybrid, SINGAPORE GROUPER IRIDOVIRUS, OCCLUSION-DERIVED VIRUS, RANA-GRYLIO-VIRUS, PROTEOMIC ANALYSIS, CAPSID PROTEINS, INVERTEBRATE IRIDOVIRUS, ENVELOPE PROTEIN, ORF 53R, CELLS, IDENTIFICATION
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Chilo iridescent virus (CIV), officially named invertebrate iridescent virus 6 (IIV6), is a nucleocytoplasmic virus with a similar to 212-kb linear dsDNA genome that encodes 215 putative open reading frames (ORFs). Proteomic analysis has revealed that the CIV virion consists of 54 virally encoded proteins. In this study, we identified the interactions between the structural proteins using the yeast two-hybrid system. We cloned 47 structural genes into both bait and prey vectors, and then analysed the interactions in Saccharomyces cerevisiae strain AH109. A total of 159 protein-protein interactions were detected between the CIV structural proteins. Only ORF 179R showed a self-association. Four structural proteins that have homologues in iridoviruses (118L, 142R, 274L and 295L) showed indirect interactions with each other. Seven proteins (138R, 142R, 361L, 378R, 395R, 415R and 453R) interacted with the major capsid protein 274L. The putative membrane protein 118L, a homologue of the frog virus 3/Ranagrylio virus 53R protein, showed direct interactions with nine other proteins (117L, 229L, 307L, 355R, 366R, 374R, 378R, 415R and 422L). The interaction between 118L and 415R was confirmed by a GST pull-down assay. These data indicate that 415R is a potential matrix protein connecting the envelope protein 118L with the major capsid protein 274L.