Investigation of antimicrobial resistance profiles of Gram negative bacteria isolated from ornamental fish aquariums in petshops Süs baliği satişi yapilan akvaryumlardan izole edilen Gram negatif bakterilerin antimikrobiyal direnç profillerinin araştirilmasi


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ÖZKAYA E. , Buruk K., KAKLIKKAYA N. , TOSUN İ.

Turk Hijyen ve Deneysel Biyoloji Dergisi, vol.74, pp.35-40, 2017 (Refereed Journals of Other Institutions) identifier

  • Publication Type: Article / Article
  • Volume: 74
  • Publication Date: 2017
  • Doi Number: 10.5505/turkhijyen.2017.18292
  • Title of Journal : Turk Hijyen ve Deneysel Biyoloji Dergisi
  • Page Numbers: pp.35-40
  • Keywords: Antimicrobial resistance, Aquarium, Gram negative

Abstract

Objective: In aquariums in shops which sell ornamental fish, a large number of fish are present together, and many microorganisms can grow in them. Moreover, since the fish which are sold are transferred with some water the microorganisms are transported to different aquariums as well. A variety of antimicrobials can be added to aquariums to prevent or treat fish diseases during the breeding of ornamental fish, and this can lead to the development of antimicrobial resistance. The aim of this study is to determine the presence and antimicrobial susceptibility of Gram negative bacteria isolated from water samples taken from ornamental fish aquariums in stores where pets are sold. Methods: Water samples were collected from 27 ornamental fish aquariums in five pet shops serving in Trabzon. These specimens were inoculated on 5% Sheep Blood Agar and Eosin-Methylene-Blue Agar media. Colonies from plaques were identified using MALDI-TOF MS. Antimicrobial susceptibility tests were performed using the Kirby-Bauer disc diffusion method. Results: A total of 31 Gram negative bacteria were identified in the 27 samples of ornamental fish aquarium water taken from the five pet shops. Citrobacter freundii was detected most frequently with a rate of 25.8%. Resistance to imipenem, meropenem and ertapenem, which are commonly used antibiotics in clinical treatments, was determined in one isolate among the four Aeromonas hydrophila isolates. Resistance to trimethoprim-sulfamethoxazole and ciprofloxacin was seen in members of Enterobactericeae, Pseudomonas spp. and Acinetobacter spp. isolated in this study. Conclusion: Fish have an important role in zoonotic transmission. Antimicrobial resistant strains can cause serious infections which have limited therapeutic options. As a result, it should be considered that contact with aquarium water may be one of the important factors in the spread of resistant microorganisms. In our study, it is the most extensive study that has been conducted to investigate the presence of antimicrobial resistance in Gram negative bacteria isolated from aquarium water.