Bioassay-guided isolation of kaempferol-3-O-beta-D-galactoside with anti-inflammatory and antinociceptive activity from the aerial part of Calluna vulgaris L.


Orhan I., Kiipeli E., Terzioglu S. , Yesilada E.

JOURNAL OF ETHNOPHARMACOLOGY, cilt.114, ss.32-37, 2007 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 114 Konu: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.jep.2007.06.017
  • Dergi Adı: JOURNAL OF ETHNOPHARMACOLOGY
  • Sayfa Sayıları: ss.32-37

Özet

Calluna vulgaris L. (Ericaceae) is used for the treatment of various inflammatory ailments in traditional medicines. In order to evaluate this ethnobotanical information, its anti-inflammatory and antinociceptive activities were studied using in vivo experimental models in mice. The ethanolic extract of the plant was first fractionated into five extracts; namely, n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol, and water fractions. Among them, the EtOAc Fr. was found to be the most effective and was further subjected to bioassay-guided fractionation and isolation procedures. After successive column chromatography applications, on Sephadex LH-20 and silica gel, a component, which is responsible for the above-mentioned activities of this species of Turkish origin, was isolated and its structure was elucidated as kaempferol-3-O-beta-D-galactoside, a common flavonol derivative by means of spectral techniques. (C) 2007 Elsevier Ireland Ltd. All rights reserved.