In this investigation, the concentration levels of potassium, calcium, iron and nickel in the three Chrysomela species were measured in the region of Erzurum (Turkey). These concentrations measured by energy dispersive X-ray fluorescence (EDXRF) spectrometry were analyzed. From these results, the difference between K, Ca, and Fe concentrations is not significant, but is significant for Ni concentrations. Therefore, the presence of an association between the determination of species and Ni concentrations levels was observed in the present study. These results are presented and discussed in this paper. (c) 2004 Elsevier Ltd. All rights reserved.