THE EFFECT OF PRE-MELATONIN TREATMENTS ON INCREASING CADMIUM STRESS TOLERANCE IN BUCKWHEAT (FAGOPYRUM ESCULENTUM MOENCH) SEEDS

Çolak N., Sungur B.

INTERNATIONAL CONFERENCE ON GLOBAL PRACTICE OF MULTIDICIPLINARY SCIENTIFIC STUDIES, Tbilisi, Georgia, 22 October 2022, pp.551

  • Publication Type: Conference Paper / Summary Text
  • City: Tbilisi
  • Country: Georgia
  • Page Numbers: pp.551
  • Karadeniz Technical University Affiliated: Yes

Abstract

Heavy metals are a group of dangerous inorganic chemicals caused contamination of agricultural land. They accumulate in soils through anthropogenic activities such as sewage disposal, fertilizer applications, industrial waste. The most common in polluted areas are lead (Pb), arsenic (As), zinc (Zn), cadmium (Cd), copper (Cu), mercury (Hg). Melatonin (Mel, N-acetyl-5-methoxytryptamine) is derived from tryptophan amino acid via the tryptamine pathway. It improves growth of seedling, enhances germination and ameliorates adversely effect of plant stress such as heavy metal and salt stresses. The present study deals with the effect of pretreatment of Mel on germination characteristics of buckwheat under Cd stress. The buckwheat seeds after pre-treatment with in 0 and 50 µM MEL were germinated in water (0), 0,25, 0,50 and 1 µM CdCl2 solutions in Petri dishes. After germination, root and shoot growths were observed and related parameters were measured for 5 days. Standard methods of analysis were used to determine germination characteristics such as germination index (GI), germination rate index (GRI), final germination percentage (FGP), coefficient of velocity of germination (CVG), relative seed growth (RSG), seed vigor index (SVI), seedling lenght, fresh (fw) and dry (dw) weights and tolerance index (TI). Increased concentrations of CdCl2 had the highest inhibitory effect on germination characteristics in buckwheat. The results showed that 1 µM CdCl2 had the lowest FGP (53) and the Mel-group had the highest rate (84). The GI, GRI, CVG, RSG, SVI, seed lenght, fresh and dry weight values decreased with increasing concenrations of CdCl2, but increased with pre-Mel treated groups in comparison to stress groups. The TI values indicated that the 50 μM Mel treatment improved the germination characteristics and enhanced the tolerance of the seedling against CdCl2 in comparison to the control plants. In conclusion, pre-treatment Mel alleviated the stress effect of CdCl2 in buckwheat seed.