In this study, Scots pine wood (Pinus sylvestris L.) powder was liquefied in the presence of pyrolytic bio-oil as a reaction medium/reagent. Firstly, the bio-oil was produced via pyrolysis of the same wood species at three different temperatures by using an extruder type pyrolyzer. Then, the wood powders were liquefied at different ratios of the wood to pyrolytic bio-oil in a sealed pressure-proof tube. The liquefaction reactions were carried out under pressure ranging between atmospheric and 8.5-MPa pressures according to the experimental conditions. The effects of the reactant ratios and the process parameters such as reaction time and temperature on the wood conversion percentage were studied. The chemical composition of the pyrolytic bio-oil and liquefied wood oil were analyzed by means of GC-MS technique. The higher heating value (HHV) and UV-Vis spectrophotometric analysis of the pyrolytic bio-oil and liquefied wood oil were also performed. The results showed that the wood powder could easily be liquefied in the pyrolytic bio-oil at different temperatures under pressure. The highest wood conversion (97.40%) was obtained at 250 degrees C for 150min at a wood to bio-oil ratio of 1: 7 with the heavy fraction of the pyrolytic bio-oil. The amount of wood residue diminished dramatically when the reaction temperature rose at the same wood to bio-oil ratio. The HHV of the liquefied wood oil was almost similar to that of the pyrolytic bio-oil. As a result, it could be inferred that the usage of pyrolytic bio-oil instead of the phenol and acid catalyst was quite efficient in the wood liquefaction process. Copyright (C) 2016 John Wiley & Sons, Ltd.