Akademik Veri Yönetim Sistemi
Farabi Tıp Dergisi, cilt.2, sa.3, ss.1-5, 2023 (Hakemli Dergi)
Irisin is a myokine with 112 amino acids and its blood concentration
is regulated by peroxisome proliferator-activated receptor-γ
coactivator1-α (PGC1-α). It is released into circulation from skeletal
muscle tissue after a photolytic cleavage of extracellular domain of
Fibronectin type III domain-containing protein 5 (FNDC5), a type I
integral membrane protein. Aprotinin is a polyvalent serin protease
inhibitor. It is added to sample solutions such as serum, plasma or
tissue extracts in order to inhibit serine proteases found in the sample
medium. Hence, degradation of the proteins to be measured can be
prevented. This study has been carried out to obtain a preliminary
data if any irisin loss could be seen in the serum samples which are
kept at -80°C for a long duration. For this purpose, blood samples of
10 men and 10 women volunteers aged between 25-40 has been
used. Aprotinin has been added to the plasma and the serum samples
have been kept at -80°C for 3 months. At the end of 3 months, irisin
levels of the samples with and without aprotinin have been
determined by ELISA. Statistical analysis has shown no difference
between the plasma samples with or without aprotinin (p=0.525).
However, a significant decrease between the serum samples with and
without aprotinin (p=0.009). In conclusion, with the results of this
study, no net decision could have been achieved to add aprotinin to
the samples for irisin determination with ELISA in plasma and
serum kept at -80°C for about 3 months.