Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae)


Yildirim H., Bekircan Ç.

JOURNAL OF INVERTEBRATE PATHOLOGY, vol.170, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 170
  • Publication Date: 2020
  • Doi Number: 10.1016/j.jip.2019.107302
  • Journal Name: JOURNAL OF INVERTEBRATE PATHOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Keywords: Altica hampei, Chrysomelidae, Diplokaryotic, Microsporidia, Nosema, Pathogen, CHAETOCNEMA-TIBIALIS COLEOPTERA, CHARACTERISTIC FEATURES, PARASITE, CERANAE
  • Karadeniz Technical University Affiliated: No

Abstract

In this study, the first microsporidian pathogen from Altica hampei (Coleoptera: Chrysomelidae) is described based on light microscopy, ultrastructural characteristics and comparative 16S SSU rDNA analysis. All developmental stages of the microsporidium are diplokaryotic and in direct contact with the host cell cytoplasm. Giemsa-stained mature spores are oval in shape and measured 3.82 +/- 0.35 mu m in length and 2.54 +/- 0.27 mu m in width. The polar filament of the binucleate spores is isofilar with 12-14 coils. Coils are 140.28 +/- 4.88 nm (135.59-147.06; n = 36) in diameter and consist of six concentric layers of different electron density and thickness. The spores have a relatively thick (161.72 +/- 29.19 nm) trilaminar spore wall. Morphological, ultrastructural and molecular features indicate that the described microsporidium belongs to the genus Nosema and is named Nosema alticae sp. nov.