Degradation of lignin by Bacillus altitudinis SL7 isolated from pulp and paper mill effluent

Khan S. I., Zarin A., Ahmed S., Hasan F., Beldüz A. O., Çanakçı S., ...Daha Fazla

WATER SCIENCE AND TECHNOLOGY, cilt.85, ss.420-432, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 85
  • Basım Tarihi: 2022
  • Doi Numarası: 10.2166/wst.2021.610
  • Dergi Adı: WATER SCIENCE AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Compendex, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.420-432
  • Anahtar Kelimeler: alkaline pH, Bacillus sp, degradation products, laccase, lignin depolymerization, SOLID-STATE FERMENTATION, KRAFT-LIGNIN, ALKALI LIGNIN, BIODEGRADATION, WASTE, INDUSTRY, LACCASE, PROTEOBACTERIUM, BIOREMEDIATION, LIGNOCELLULOSE
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Lignin is a major by-product of pulp and paper industries, which is resistant to depolymerization due to its heterogeneous structure. Degradation of lignin can be achieved by the use of potential lignin-degrading bacteria. The current study was designed to evaluate the degradation efficiency of newly isolated Bacillus altitudinis SL7 from pulp and paper mill effluent. The degradation efficiency of B. altitudinis SL7 was determined by color reduction, r lignin contents, and ligninolytic activity from degradation medium supplemented with alkali lignin (3 g/L). B. altitudinis SL7 reduced color and lignin contents by 26 and 44%, respectively, on 5th day of the incubation, as evident from the maximum laccase activity. Optimum degradation was observed at 40 degrees C and pH 8.0. FT-IR spectroscopy and GC-MS analysis confirmed lignin degradation by emergence of the new peaks and identification of low molecular weight compounds in treated samples. The identified compounds such as vanillin, 2-methyoxyhenol, 3-methyl phenol, oxalic acid and ferulic acid suggested the degradation of coniferyl and sinapyl groups of lignin. Degradation efficiency of B. altitudinis SL7 towards high lignin concentration under alkaline pH indicated the potential application of this isolate in biological treatment of the lignin-containing effluents.