Effects of 7,8 DHF on Sensory Motor Performance in Cafeteria Diet

Creative Commons License

Keser H., Şahin E., Abidin S., Alver A., Abidin İ.

5th International 34th National Biophysics Congress, İzmir, Türkiye, 6 - 09 Eylül 2023, ss.233

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.233
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Aim: Cafeteria diet has been suggested to induce obesity. It consists of delicious, over-processed unhealthy food products with high fat and sugar content. 7,8-Dihydroxyflavone (DHF) is an antioxidant flavonoid that can cross the blood-brain barrier and mimic brain-derived neurotrophic factor (BDNF). The aim of the study is to determine the possible protective effects of 7,8-DHF against cafeteria diet-induced damages of sensory motor functions by behavioral tests.

Methods: Male C57BL/6 mice were divided into four groups (n=9-11) as control (K), cafeteria diet (KD), cafeteria diet+vehicle (KD-ÇÖZ) and cafeteria diet+7,8-Dihydroxyflavone (KD-DHF). The cafeteria diet consists of a mixture of different snacks such as biscuits, crackers, fruitcakes, chips, and wafers. K group mice were fed with control diet and other groups were fed with cafeteria diet for 16 weeks. 5 mg/kg 7,8 DHF was administered to the KD-DHF group and 17% DMSO was administered to KD-ÇÖZ group intraperitoneally at the last 28 days. Then cylinder test, hang wire test, adhesive removal tests were performed to test sensory-motor functions. Scoring and analysis were confirmed by the offline evaluation of video recordings. Statistical analysis were made by one way ANOVA test and Tukey post hoc test was used.

Results: KD-DHF group performed higher than KD group in hangwire and adhesive removal tests. In the adhesive removal tests, the difference between the groups was significant (p <0.05). In addition, it was observed that the performance of the KD group was lower than that of the K group (p <0.05).

Conclusion: Performed tests determines postural coordination, muscle strength, responding to touch stimuli due to sensory motor cortex functions. Cafeteria diet may cause dysfunctions at this region depends on BDNF receptor TrkB activation. According to our results, 7,8 DHF administration may have a protective role against cafeteria diet-induced sensory motor disorders.