Akademik Veri Yönetim Sistemi
Federation of European Neuroscience Societies (FENS) 2024 Forum of Neuroscience, Vienna, Avusturya, 25 - 29 Haziran 2024, ss.1
Neuropeptide S is a 20 amino acid neuromodulator and it is the physiological ligand of Neuropeptide S receptor, NPSR. NPSR activates intracellular pathways that control long-term gene expression as well as PLC-Calcium pathway. The expression of the NPS receptor in olfactory regions like piriform cortex, suggests that there may be a functional relationship between NPS and epileptic activity.
The aim of this study was to investigate the effects of suppression of the NPS receptor pathway on epileptiform activity in piriform cortex. Acute brain slices were obtained from 6-8 weeks old C57BL/6 mice. Horizontal slices including the piriform areas used for electrophysiological recordings. Two glass micropipettes were placed on layer 2 and electrical activities were recorded simultaneously from two different channels. Artificial epilepsy like activity was initiated by bath application of 4AP (100 µM). To reveal the effect of NPSR, SHA 68 (20 µM), a NPRS antagonist, was applied during epileptic activity. Frequencies, durations and amplitudes of short lasting (< 4 s, inter-ictal) and longer lasting (>4 s, ictal) events were compared. The changes of correlation coefficient of the two recordings were also calculated.
Recordings from 10 piriform cortices that showed epileptic activity were analyzed. SHA 68 application decreased the frequency of inter-ictal events (p<0.05) and the total power of oscillatory activity (p<0.01).
Piriform cortex is the major cortical area of the olfactory system. It is also critical for the initiation of epileptic activities. Neuropeptide S signaling may contribute to the synchronization of epileptiform activity in piriform cortex.