This paper comprises mitotic chromosome numbers of 37 accessions representing 24 taxa and 1 interspecific hybrid of Cirsium from north-east Anatolia. Twelve counts are new to science, and eighteen counts and one new ploidy level are reported for the first time from Turkey. Other counts are the confirmations of limited or unique previous data. In the present study, the chromosome numbers of the studied taxa are determined as 2n = 34, 36 and 68 respectively. Moreover, B chromosomes are reported in two taxa. The relevance of the results are also discussed in relation to the available data for each of the analysed taxa.