This study presents findings retrieved from an analysis of the Turkish Biology Curriculum Guidelines and it's the alignment to the Student Selection Examination (SSE). Webb's ( 2007) alignment criteria were used to investigate relationship among guidelines and assessment. In this process, biology questions of SSE were examined in detail with ten biology teachers. The results showed that although alignment consistency was high according to the depth-of-knowledge consistency and categorical concurrence criterions, the SSE questions and curriculum outcomes were not fully aligned considering low consistency of range of knowledge and balance of representation criterions. Consequently, validity and reliability of the examination for higher education eligibility need to be increased starting from analysis the contents areas and the nature of questions. (c) 2011 Published by Elsevier Ltd.