Chromium isotope data were obtained from olivine, orthopyroxene and chromite separates of the Kizildag ophiolite, SE Turkey, to investigate the effects of high temperature magma processes on Cr isotope fractionation. Harzburgite in the Kizildag ophiolite has the delta Cr-53 values of -0.14 to -0.12 parts per thousand in chromite, and -0.08 to -0.01 parts per thousand in bulk rocks. These Cr isotope fractionations could be driven by partial melting and metasomatism. The dunite and chromitite samples from the mantle-crust transition zone of the Kizildag ophiolite are cumulates, and their chromite and olivine have delta Cr-53 values of -0.29 to -0.06 parts per thousand and -0.11 to 0.41 parts per thousand, respectively. The delta Cr-53 values of chromite are negatively correlated with the chemical indices of fractional crystallization (e.g., Fe# of chromite), suggesting that Cr isotopes were fractionated during fractional crystallization. In the chromitite, the degree of Cr isotope fractionation increases with fractional crystallization, with 0.23 parts per thousand Cr isotope fractionation occurring at mineral scale. During fractional crystallization, solid phases preferentially incorporate Cr, particularly heavy Cr isotopes, driving the depletion of Cr and enrichment of light Cr isotopes in the evolved melts. The delta Cr-53 values of olivine are higher than the coexisting chromite, which could be explained by the fact that the olivine grains were probably formed earlier than the chromite. The Cr isotopic features of chromite in the podiform chromitite (-0.22 to 0.04 parts per thousand) from the mantle sequence are consistent with the modelled fractional crystallization trend, confirming the magmatic origin of the podiform chromitite. Therefore, a significant Cr isotope fractionation at chromite from the Kizildag ophiolite could be induced by the high-temperature fractional crystallization. (C) 2019 Elsevier B.V. All rights reserved.